Stimulus of leucine incorporation into perfused liver protein by insulin
- 1 January 1963
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Legacy Content
- Vol. 204 (1), 140-142
- https://doi.org/10.1152/ajplegacy.1963.204.1.140
Abstract
Livers from 24-hr fasting sham-operated or partially pancreatectomized rats were perfused at 38 C with heparinized blood from normal 24-hr fasting rats. Leucine-1-C14, 1.6 x 105 counts/min per ml, and glucose to make the initial total concentration 2 mg/ml were added to the blood. Incorporation of leucine-1-C14 into protein proceeded linearly for 2 hr, then at a decreased rate. The leucine-1-C14 incorporated into protein over the initial 2-hr perfusion period decreased progressively as the time after partial pancreatectomy of the liver donors increased, up to 6 months. Insulin, 0.01 unit/ml, added to the perfusing blood stimulated leucine incorporation into protein of livers from rats partially pancreatectomized 3 months before (mean terminal blood sugar 160 mg/100 ml) but not into that of livers from the 6-month series (mean terminal blood sugar 315 mg/100 ml). No significant effect of insulin was obtained in any of the sham-operated groups. It is concluded that insulin added in vitro can stimulate incorporation of leucine into protein in livers from mildly diabetic, but not severely diabetic, rats.This publication has 4 references indexed in Scilit:
- Insulin stimulus of leucine incorporation in rat liver proteinAmerican Journal of Physiology-Legacy Content, 1962
- Portal venous injection of insulin in the diabetic rat: time of induction of changes in hepatic lipogenesis, cholesterogenesis, and glycogenesisJournal of Lipid Research, 1960
- Effect of glucagon on metabolism of glucose and acetate by isolated rat liverAmerican Journal of Physiology-Legacy Content, 1959
- Alloxan Diabetes and Demonstrated Direct Action of Insulin on Metabolism of Isolated Perfused Rat LiverAmerican Journal of Physiology-Legacy Content, 1957