Laying hens were fed a diet that contained either 0.10 mg/kg natural selenium, 0.10 mg/kg natural selenium plus 0.32 mg/kg selenite selenium, or 0.42 mg/kg natural selenium. After the hens had been fed these diets for 180 days, tissues and eggs from hens fed 0.42 mg/kg natural selenium contained significantly more selenium than tissues and eggs from hens fed the selenite selenium. Only a small percentage of liver selenium resulting from the feeding of natural selenium compounds could be removed by dialysis in a pH 11 solution, but practically all of it resulting from selenite feeding could be removed. A second experiment showed that when hens were fed 0.1 mg/kg of selenite selenium, significantly more selenium was present in the yolk than in the white of the egg. Selenium in the white resulting from the selenite feeding could be removed by dialysis but not that in the yolk.