Separation and determination of cefpiramide in human plasma by electrokinetic chromatography with a micellar solution and an open tubular-fused silica capillary.

Abstract

Electrokinetic chromatography was used to determine cefpiramide (CPM) in human plasma, using antipyrine (AP) as an internal standard. A plasma sample was introduced into a fused silica capillary. Then high voltage was applied between the ends of the capillary dipped in a phosphate buffer solution containing sodium dodecyl sulfate above critical micellar concentration (CMC). The CPM and AP were separated from each other and from plasma proteins within 5 min. The calibration line indicated good linearity in the concentration range over 10 to 300 .mu.g/ml.