Multiple-column solid-phase glycopeptide synthesis

Abstract

The preparation of the two new building blocks N^α-Fmoc-Ser(Ac₃-α-D-GalpNAc)-OPfp 6 and N^α-Fmoc-Thr(Ac₃-α-D-GalpNAc)-OPfp 7 and their application in a simultaneous multiple-column solid-phase synthesis of 40 different O-glycopeptides from human intestinal mucin and porcine submaxillary gland mucin are described. All glycopeptides were obtained in excellent purity and were characterised by 1D- and 2D-¹H NMR spectroscopy and amino acid analyses.