A Monoclonal Antibody Specific for the Carboxy-Terminus of Angiotensin II
- 1 January 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 3 (4), 373-376
- https://doi.org/10.1089/hyb.1984.3.373
Abstract
A monoclonal antibody specific for the carboxy-terminus of angiotensin II was produced from the somatic cell fusion between SP2/0 myeloma cells and spleen cells from CAF/J mice immunized with angiotensin II selectively coupled to thyroglobulin. The subcloned line D5-7R2 was augmented in the ascites form and shown to be specific for angiotensin II (octapeptide) and shorter angiotensin peptides. This antibody did not cross-react with angiotensin I (decapeptide). This selectivity will be important in clinical assays that measure angiotensin II in the presence of angiotensin I.This publication has 9 references indexed in Scilit:
- Monoclonal Antibodies to a Synthetic Fibrin-Like Peptide Bind to Human Fibrin But Not FibrinogenScience, 1983
- Selectivity of angiotensin II antiseraJournal of Immunological Methods, 1983
- An antiidiotypic antibody that recognizes the beta-adrenergic receptor.JCI Insight, 1982
- Production of monoclonal antibodies: Strategy and tacticsJournal of Immunological Methods, 1980
- The Effect of Captopril on Blood Pressure and Angiotensins I, II and III in Sodium-Depleted Dogs: Problems Associated with the Measurement of Angiotensin II after Inhibition of Converting EnzymeClinical Science, 1980
- A better cell line for making hybridomas secreting specific antibodiesNature, 1978
- Application of a Radioimmunoassay for Angiotensin I to the Physiologic Measurements of Plasma Renin Activity in Normal Human Subjects1Journal of Clinical Endocrinology & Metabolism, 1969
- Solid-Phase Radioimmunoassay of Estradiol-17βJournal of Clinical Endocrinology & Metabolism, 1969
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964