Analysis of Sequence Transfers Resembling Gene Conversion in a Mouse Antibody Transgene
- 9 September 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 265 (5178), 1590-1593
- https://doi.org/10.1126/science.8079173
Abstract
The role of gene conversion in murine immunoglobulin gene diversification is unclear. An antibody gene construct designed to provide the homologous donor and acceptor sequences required for conversion mechanisms was produced and used to generate transgenic mice. When these transgenic mice were immunized, DNA sequence transfers between tandem transgene VDJ regions were detectable and resembled gene conversion events. There is a strong link between these conversion-like sequence transfers and transgene somatic hypermutation, suggesting that both processes might occur at the same stage of B cell differentiation.Keywords
This publication has 9 references indexed in Scilit:
- Somatic hypermutation of an immunoglobulin mu heavy chain transgene.The Journal of Experimental Medicine, 1993
- Isotype switching of an immunoglobulin heavy chain transgene occurs by DNA recombination between different chromosomesCell, 1990
- Parallel evolution of antibody variable regions by somatic processes: consecutive shared somatic alterations in VH genes expressed by independently generated hybridomas apparently acquired by point mutation and selection rather than by gene conversion.The Journal of Experimental Medicine, 1990
- Molecular basis of the allelic inheritance of rabbit immunoglobulin VH allotypes: Implications for the generation of antibody diversityCell, 1990
- Point mutations cause the somatic diversification of IgM and IgG2a antiphosphorylcholine antibodies.The Journal of Experimental Medicine, 1988
- A hyperconversion mechanism generates the chicken light chain preimmune repertoireCell, 1987
- Palindromic sequences are associated with sites of DNA breakage during gene conversionNucleic Acids Research, 1986
- Affinity analysis of idiotype-positive and idiotype-negative Ars-binding hybridoma proteins and Ars-immune seraMolecular Immunology, 1983
- A single VH gene segment encodes the immune response to phosphorylcholine: Somatic mutation is correlated with the class of the antibodyCell, 1981