Matrix‐assisted laser desorption ionization with a magnetic mass spectrometer
- 1 September 1991
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 5 (9), 395-399
- https://doi.org/10.1002/rcm.1290050905
Abstract
Matrix-assisted laser desorption ionization has been carried out with a high mass double-focusing magnetic mass spectrometer. The pulsed ion signal, generated by irradiation of the sample (substance P, ubiquitin, and cytochrome c) embedded in 2,5-dihydroxybezoic acid with a XeF excimer laser (353 nm, 12 ns pulse, 10–160 Hz), was recorded with an integrating array detector. Good resolution of 2600 (full width at half maximum) and high sensitivity (a few pmol) were obtained. The loss of small neutral fragments was observed, supporting the notion that the peak broadening observed in the time-of flight mass spectrometers commonly used for this ionization mode is due to such metastable decomposition.Keywords
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