Cell fate-specific regulation of EGF receptor trafficking during Caenorhabditis elegans vulval development
Open Access
- 11 May 2006
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 25 (11), 2347-2357
- https://doi.org/10.1038/sj.emboj.7601137
Abstract
By controlling the subcellular localization of growth factor receptors, cells can modulate the activity of intracellular signal transduction pathways. During Caenorhabditis elegans vulval development, a ternary complex consisting of the LIN‐7, LIN‐2 and LIN‐10 PDZ domain proteins localizes the epidermal growth factor receptor (EGFR) to the basolateral compartment of the vulval precursor cells (VPCs) to allow efficient receptor activation by the inductive EGF signal from the anchor cell. We have identified EGFR substrate protein‐8 (EPS‐8) as a novel component of the EGFR localization complex that links receptor trafficking to cell fate specification. EPS‐8 expression is upregulated in the primary VPCs, where it creates a positive feedback loop in the EGFR/RAS/MAPK pathway. The membrane‐associated guanylate kinase LIN‐2 recruits EPS‐8 into the receptor localization complex to retain the EGFR on the basolateral plasma membrane, and thus allow maximal receptor activation in the primary cell lineage. Low levels of EPS‐8 in the neighboring secondary VPCs result in the rapid degradation of the EGFR, allowing these cells to adopt the secondary cell fate. Extracellular signals thus regulate EGFR trafficking in a cell type‐specific manner to control pattern formation during organogenesis.Keywords
This publication has 55 references indexed in Scilit:
- Distinct roles for Mind bomb, Neuralized and Epsin in mediating DSL endocytosis and signaling inDrosophilaDevelopment, 2005
- EGF Signal Propagation during C. elegans Vulval Development Mediated by ROM-1 RhomboidPLoS Biology, 2004
- The tetrameric L27 domain complex as an organization platform for supramolecular assembliesNature Structural & Molecular Biology, 2004
- ErbB Receptors: Directing Key Signaling Networks Throughout LifeAnnual Review of Pharmacology and Toxicology, 2004
- C. elegans: des neurones et des gènesmédecine/sciences, 2003
- The Epidermal Growth Factor Receptor Juxtamembrane Domain Has Multiple Basolateral Plasma Membrane Localization Determinants, Including a Dominant Signal with a Polyproline CorePublished by Elsevier ,2002
- Coordinated Folding and Association of the LIN-2, -7 (L27) Domain: AN OBLIGATE HETERODIMERIZATION MODULE INVOLVED IN ASSEMBLY OF SIGNALING AND CELL POLARITY COMPLEXESJournal of Biological Chemistry, 2002
- Regulation of the tyrosine kinase substrate Eps8 expression by growth factors, v-Src and terminal differentiationOncogene, 1997
- The lin-12 locus specifies cell fates in caenorhabditis elegansCell, 1983
- Alterations in cell lineage following laser ablation of cells in the somatic gonad of Caenorhabditis elegansDevelopmental Biology, 1981