Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde
- 1 January 2005
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 45 (5), 455-459
- https://doi.org/10.1002/em.20117
Abstract
Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site‐specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5–6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals. Environ. Mol. Mutagen., 2005.Keywords
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