Fragmentation of Rat Serum Albumin by Cyanogen Bromide Cleavage and the Amino Acid Sequences of Four Fragments1

Abstract

Rat serum albumin was cleaved into seven peptides by cyanogen bromide treatment followed by reduction and carboxymethylation. The amino acid composition and the N-terminus of each peptide and the amino acid sequences of four peptides were determined by conventional methods. An alignment of these peptides in the original protein was proposed in the light of the established sequences of bovine and human plasma albumin.