Sedimentation of wormlike coils. II
- 15 September 1980
- journal article
- research article
- Published by AIP Publishing in The Journal of Chemical Physics
- Vol. 73 (6), 3007-3009
- https://doi.org/10.1063/1.440433
Abstract
An application of the theories of Hearst and Stockmayer for the sedimentation coefficient of wormlike coils of length shorter than 2.2 Kuhn statistical lengths, and Gray, Bloomfield and Hearst for longer wormlike coils with excluded volume to recent sedimentation data on homogeneous DNA samples is presented. The data is entirely consistent with the predictions of the theories. The molecular parameters obtained from the analysis of the data are a Kuhn statistical length at 0.2 ionic strength of 1150 Å; at 0.1 ionic strength of 1290 Å; and a chain backbone diameter of 20–30 Å.Keywords
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