Separation of Methylated Purines by High Pressure Liquid Chromatography
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 1 (1), 75-88
- https://doi.org/10.1080/01483917808068379
Abstract
This paper reports a method for the separation and measurement of methylated purines of interest to carcinogenesis studies by high-pressure liquid chromatography (HPLC) following their column chromatographic isolation from collected urine samples. HPLC was evaluated on three different cation-exchange columns, with optimum conditions obtained on a Partisil 10-SCX column employing isocratic elution with 0.25M (NH4)H2PO4 at pH 4.0. This column was also found to be useful for the separation of mono-methylguanine isomers. Application is shown to the analysis of rat urine following animal treatment with methyl methanesulfonate.Keywords
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