Cleavage at the twelve-base-pair sequence 5′-TCTAGATCTAGA-3′ using M·Xbal(TCTAGm6A) methylation andDpnl(Gm6A/TC) cleavage
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (6), 1603-1607
- https://doi.org/10.1093/nar/18.6.1603
Abstract
The DNA methylase M·Xbal was isolated from an E. coli recombinant clone. We deduce that the enzyme methylates at the sequence 5′-TCTAGm6A-3′. In combination with the methyiation-dependent restriction endonuclease, Dpnl (5′-Gm6A/TC-3′), DNA cleavage occurs at the sequence 5′-TCTAGA/TCTAGA-3′. This twelve-base-pair site should occur once every 16,000,000 base pairs in a random sequence of DNA. The exceptional rarity of the M·Xbal/Dpnl sequence makes it an ideal candidate for transpositional integration of a unique cleavage site into bacterial genomes. Retrotransposition into mammalian genomes is also an attractive possibility.This publication has 23 references indexed in Scilit:
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