RESTRICTION ENDONUCLEASE MAPPING OF GAMMA-DELTA-BETA-GLOBIN REGION IN G-GAMMA(BETA)+ HPFH AND A CHINESE A-GAMMA-HPFH VARIANT
- 1 January 1983
- journal article
- research article
- Vol. 35 (4), 611-620
Abstract
Restriction endonuclease mapping of the .beta.-globin genomic region was used for studying the molecular basis of 2 variants of hereditary persistence of fetal Hb (HPFH): an African G.gamma.(.beta.)+ HPFH and a Chinese HPFH variant with predominant synthesis of A.gamma. chains. HPFH and control DNA samples were digested with a battery of restriction enzymes, and the fragments were identified by hybridization to a family of discrete probes. DNA fragments from the A.gamma. HPFH (Chinese) and the G.gamma.(.beta.)+ HPFH individuals were identical with those of the normal controls. The 2 mutants may be the result of small structural anomalies of DNA sequences that play a role in the regulation of the expression of .gamma.-globulin genes.This publication has 44 references indexed in Scilit:
- A gene deletion ending at the midpoint of a repetitive DNA sequence in one form of hereditary persistence of fetal haemoglobinNature, 1982
- Restriction endonuclease maps of the β-like globin gene cluster in the British and Greek forms of HPFH, and for one example ofGγβ+HPFHBritish Journal of Haematology, 1982
- Linkage Analysis of Nondeletion Hereditary Persistence of Fetal HemoglobinScience, 1982
- Complete nucleotide sequence of the human δ-globin geneCell, 1980
- Heterogeneity in the molecular basis of hereditary persistence of fetal haemoglobinNature, 1980
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Abnormal or absent β mRNA in β0 Ferrara and gene deletion in δβ thalassaemiaNature, 1976
- δβ-Thalassemia is due to a gene deletionCell, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Globin Chain Synthesis in the Greek Type (Aγ) of Hereditary Persistence of Fetal HaemoglobinBritish Journal of Haematology, 1975