Chromatographic and Electrophoretic Separation of Creatine Kinase Isoenzymes Compared

Abstract

We compared two techniques for separating and evaluating serum creatine kinase isoenzymes—fluorometric agarose electrophoresis and Sephadex chromatography—in 50 patients, 25 of whom had confirmed acute myocardial infarction. In every case isoenzyme MB (heart isoenzyme) was detected with equal sensitivity by either procedure. Evidently, only the presence or absence of MB is clinically significant; none of the 25 patients without infarction had detectable MB activity in their serum. Columns connected to a continuous-flow sample line for analyses of the eluting stream without further modification produced satisfactory results