Coumarin in Chromosome Analysis

Abstract

Root tips of monocotyledons were soaked 2.5-3.0 hours at 25–27° C. in saturated aqueous coumarin solution and stained in a mixture of N HC 1 and 2% aceto-orcein (1:9 by volume) 3-4 seconds over a flame. They were then squashed in 1% orcein under a cover glass, the excess stain blotted and the cover sealed. Preparations could be kept about one week. Good chromosome morphology was secured.