Photoinactivation of Detergent-Solubilized Plasma Membrane ATPase from Rosa damascena
- 1 March 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 74 (3), 617-621
- https://doi.org/10.1104/pp.74.3.617
Abstract
The photochemistry of vesicular and detergent-solubilized preparations of plasma membrane-associated ATPase was investigated in R. damascena. The cholate-solubilized ATPase activity fractionated into 2 peaks on Sephadex G-150 column with simple, but different UV sensitivities. The larger enzyme was UV sensitive; the smaller enzyme was relatively insensitive. The activity of both ATPase fractions depended on environment: both were inactive in cholate, relatively inactive in phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol and active in phosphatidylglycerol and phosphatidylserine. The UV sensitivities of both fractions also depended on their environment. For the UV sensitive fraction, the action spectrum differed in the 300-400 nm range when the fraction was irradiated with and without lipids. For the resistant fraction, UV sensitivity at 290 nm differed (up to 6-fold) in different lipids. The resistant fraction solubilized in octylglucoside had an action spectrum very different from that in cholate or in lipid vesicles. The absorption spectra of the different preparations reflected the action spectra. For both UV sensitive and insensitive fractions, the action spectra for photoactivation had peaks at 290 nm, suggesting that the chromophores were tryptophanyl residues. The loss of ATPase activity was strictly correlated with the loss of fluorescence from tryptophan in the partially purified enzymes. Cs+ protected the UV sensitive activity but not the insensitive one. A model which explains the difference in UV sensitivities, based on the positions of the tryptophan residues in the 2 proteins, is proposed.This publication has 13 references indexed in Scilit:
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