A PROBABLE SOURCE OF ERRORS IN ELECTRON-HISTOCHEMISTRY

Abstract

The postfixation in OsO4 of frozen sections of mouse kidney treated by the Gomori methods (calcium-cobalt and calcium-lead) for the demonstration of the alkaline phosphatase activity, gradually removes the final product of the reaction (phosphate or sulfide of cobalt or lead). By prolonging the duration of the postfixation a completely negative reaction is obtained. These observations are of probable importance in electron histochemistry.