THE CHARACTERIZATION OF A NEW SPECIES OF LEUCYL-sRNA FORMED DURING METHIONINE DEPRIVATION OF ESCHERICHIA COLI WITH RELAXED CONTROL
- 1 March 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 53 (3), 549-556
- https://doi.org/10.1073/pnas.53.3.549
Abstract
Analysis by chromatog-raphy on methylated albumin columns has provided a device for the characterization of a unique species of leucine-sRNA that accumulates during relaxed control. By the use of various related mutants of E. coli, evidence was obtained indicating that the formation of this species of leucyl-sRNA is the result of de novo synthesis of sRNA in the absence of the methyl donor ordinarily used to complete the synthesis of methylated bases at the polynucleotide level.Keywords
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