Modification of the laminin α4 chain by chondroitin sulfate attachment to its N‐terminal domain
- 27 August 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 505 (1), 173-178
- https://doi.org/10.1016/s0014-5793(01)02812-5
Abstract
The N-terminal domain of laminin alpha 4 chains corresponds to a short rod-like structure which after recombinant production was found to be modified by chondroitin sulfate. Substitution occurred mainly to a single serine in its N-terminal ASGDG sequence. A similar yet partial modification was also demonstrated for the alpha 4 chain present in extracts of adult mouse tissues. Antibodies to the fragment were useful to demonstrate a relatively high content of alpha 4 in several tissues and for the immunolocalization in various blood vessels, some basement membranes and interstitial regions.Keywords
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