1,2‐Diacylglycerol accumulation in human neutrophils does not correlate with respiratory burst activation
- 30 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 243 (2), 399-403
- https://doi.org/10.1016/0014-5793(89)80170-x
Abstract
Measurements of the level of 1,2-diacylglycerol (1,2-DG during activation of the respiratory burst of human neutrophils by formyl-methionyl-leucyl-phenylalanine (fMLP in the presence of platelet-activating factor (PAF or by opsonized particles show that a correlation between accumulation of 1,2-DG and O2 consumption does not exist. Inhibition of protein kinase C activity with staurosporine before addition of opsonized particles demonstrates that the first phase of the respiratory burst is not inhibited, whereas the second phase, which is accompanied by a rise in the content of 1,2-DG, is strongly inhibited. This study indicates that accumulation of 1,2-DG cannot be the sole signal for the initiation of the respiratory burst in human neutrophils.Keywords
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