Development of Multianalyte Sensor Arrays Composed of Chemically Derivatized Polymeric Microspheres Localized in Micromachined Cavities
- 21 February 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 123 (11), 2559-2570
- https://doi.org/10.1021/ja003341l
Abstract
The development of a chip-based sensor array composed of individually addressable polystyrene−poly(ethylene glycol) and agarose microspheres has been demonstrated. The microspheres are selectively arranged in micromachined cavities localized on silicon wafers. These cavities are created with an anisotropic etch and serve as miniaturized reaction vessels and analysis chambers. A single drop of fluid provides sufficient analysis media to complete ∼100 assays in these microetch pits. The cavities possess pyramidal pit shapes with trans-wafer openings that allows for both fluid flow through the microreactors/analysis chambers and optical access to the chemically sensitive microspheres. Identification and quantitation of analytes occurs via colorimetric and fluorescence changes to receptor and indicator molecules that are covalently attached to termination sites on the polymeric microspheres. Spectral data are extracted from the array efficiently using a charge-coupled device allowing for the near-real-time digital analysis of complex fluids. The power and utility of this new microbead array detection methodology is demonstrated here for the analysis of complex fluids containing a variety of important classes of analytes including acids, bases, metal cations, metabolic cofactors, and antibody reagents.Keywords
This publication has 48 references indexed in Scilit:
- Enzymatic determination of ethanol and glycerol by flow injection parallel multi-site detectionAnalytica Chimica Acta, 2000
- Complexometric Titrations: Competition of Complexing Agents in the Determination of Water Hardness with EDTAJournal of Chemical Education, 1997
- Gas identification by modulating temperatures of SnO2-based thick film sensorsSensors and Actuators B: Chemical, 1997
- Congo red- and Zn(II)-derivatized monosize poly(MMA-HEMA) microspheres as specific sorbent in metal chelate affinity of albuminJournal of Applied Polymer Science, 1997
- Array-Based Vapor Sensing Using Chemically Sensitive, Carbon Black−Polymer ResistorsChemistry of Materials, 1996
- Empirically defined regions of influence for clustering analysesPattern Recognition, 1995
- Synthesis and characteristics of microspheres of polystyrene derivativesJournal of Polymer Science Part A: Polymer Chemistry, 1986
- Enzymatic determination of glucose in a flow system by catalytic oxidation of the nicotinamide coenzyme at a modified electrodeAnalytica Chimica Acta, 1985
- Compleximetric indicators: characteristics and applicationsPublished by Walter de Gruyter GmbH ,1983
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963