Vestibular Compensation Affects Endogenous Phosphorylation of Frog Brain Proteins
- 1 January 1992
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 58 (1), 65-71
- https://doi.org/10.1111/j.1471-4159.1992.tb09277.x
Abstract
The effect of unilateral labyrinthectomy followed by the process of vestibular compensation on the incorporation of radioactive phosphate into frog brain proteins was investigated. Phosphoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography. The present data show that unilateral labyrinthectomy affects the incorporation of 32P into various frog brain proteins. In particular, the phosphorylation of a 20-kDa protein appeared enhanced during early stages of vestibular compensation (4-12 days). This 20-kDa protein was shown to be immunologically related to myelin basic protein and its phosphorylation was regulated by an endogenous calcium/calmodulin-dependent protein kinase. These data might indicate that in addition to neuronal components, components of glial origin are also involved in biochemical events that lead to functional recovery after neuronal lesions.Keywords
This publication has 33 references indexed in Scilit:
- A membrane phosphoprotein associated with neural development, axonal regeneration, phospholipid metabolism, and synaptic plasticityTrends in Neurosciences, 1987
- Phylogenetic examination of vertebrate central nervous system myelin proteins by electro-immunoblottingNeuroscience Letters, 1985
- Regulation of Phosphate Incorporation into Four Brain Phosphoproteins that Are Affected by ExperienceJournal of Neurochemistry, 1985
- In vivo Phosphorylation of Myelin Basic Proteins in Developing Mouse Brain: Evidence that Phosphorylation Is an Early Event in Myelin FormationDevelopmental Neuroscience, 1983
- Basic Protein in Brain Myelin Is Phosphorylated by Endogenous Phospholipid‐Sensitive Ca2+‐Dependent Protein KinaseJournal of Neurochemistry, 1982
- Ca2+-Calmodulin dependent phosphorylation of myelin isolated from rabbit brainBiochemical and Biophysical Research Communications, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Ergebnisse der Durchschneidung des N. acusticus, nebst Erörterung der Bedeutung der semicirculären Canäle für das KörpergleichgewichtPflügers Archiv - European Journal of Physiology, 1883