Localization of Lipoxygenases 1 and 2 in Germinating Soybean Seeds by an Indirect Immunofluorescence Technique

Abstract

The effect of CN⁻ and N₂ on the electrical membrane potential (E_m) was compared with that of CN⁻ on the ATP levels in cotyledons of Gossypium hirsutum and in Lemna gibba L. In mature cotton tissue, CN⁻ depolarized E_m to the energy-independent diffusion potential (E_D) in the dark. In the light E_m recovered transiently. The same was observed in leaves of Nicotiana, Avena, Impatiens, Kalanchoë, and in Lemna. In contrast, in young cotton cotyledons and tobacco leaves and, to a large extent, in +sucrose-grown Lemna, E_m was depolarized to E_D also in the light in a similar way as in the dark.