Regulatory nucleotides involved in the Rel function of Bacillus subtilis
- 1 November 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 140 (2), 671-679
- https://doi.org/10.1128/jb.140.2.671-679.1979
Abstract
Accumulation of polyphosphorylated nucleotides in B. subtilis in relation to the function of the rel gene was examined. During inhibition of isoleucine activation by O-methylthreonine, wild-type B. subtilis cells accumulate unusual nucleotides with the chromatographic and chemical properties of pppApp, ppApp, pppGpp, ppGpp, pGpp and ppGp. During the C source downshift elicited by inhibiting glucose uptake, accumulation of the polyphosphorylated guanosine but not adenosine nucleotides was observed. At the end of log phase in sporulation medium, a small transient accumulation of the polyphosphorylated guanosine but not adenosine nucleotides was observed. No nucleotide with chromatographic behavior expected for pppAppp could be detected under any conditions. The rel mutant of Swanton and Edlin did not accumulate any of these polyphosphorylated nucleotides under any conditions examined. The rel mutant is unimpaired in sporulation. One or more of the nucleotides detected may be involved in controlling specificity of transcription during the stringent response but none are required for sporogenesis.This publication has 17 references indexed in Scilit:
- A new nucleotide involved in the stringent response in Escherichia coli. Guanosine 5‘-diphosphate-3‘-monophosphate.Journal of Biological Chemistry, 1979
- Studies on the Control of DevelopmentEuropean Journal of Biochemistry, 1978
- The role of energy-generating processes in the degradation of guanosine tetraphosphate, ppGpp, in Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Studies on the Control of DevelopmentEuropean Journal of Biochemistry, 1976
- Control of development: role of regulatory nucleotides synthesized by membranes of Bacillus subtilis in initiation of sporulation.Proceedings of the National Academy of Sciences, 1976
- REGULATION OF BACTERIAL ppGpp AND pppGppAnnual Review of Microbiology, 1975
- Thermolability of the stringent factor in rel mutants of Escherichia coliJournal of Molecular Biology, 1973
- MSI and MSII made on Ribosome in Idling Step of Protein SynthesisNature, 1972
- Isolation and characterization of an RNA relaxed mutant of B. subtilisBiochemical and Biophysical Research Communications, 1972
- [12] The use of the borate complexPublished by Elsevier ,1967