Early Effect of Testosterone in the Incorporation of Uridine into RNA of Mouse Kidney and Seminal Vesicles1

Abstract

The incorporation of [5–³H]—uridine and [5–³H]—cytidine into the acid soluble fraction and RNA of the mouse kidney and seminal vesicles was not influenced by castration. The administration of testosterone, however, stimulated within 12 hr an increased incorporation of [5–³H]—uridine. The continued administration of the androgen resulted in a disappearance of this effect within 48 hr in the kidney and over a period of several days in the seminal vesicles. A high dose (5 μmoles) of the uridine elicited a greater response than a tracer dose. The effect of testosterone on uridine incorporation was opposite to that previously observed with orotic acid and indicates that testosterone stimulates the salvage pathway for RNA synthesis in the seminal vesicles and the kidneys. (Endocrinology92: 917, 1973)