BIOLOGICAL-ACTIVITY OF CHEMICALLY SYNTHESIZED ANALOGS OF LIPID-A - DEMONSTRATION OF ADJUVANT EFFECT IN HAPTEN-SENSITIZED LIPOSOMAL SYSTEM

Abstract

The adjuvant effect of synthetic analogs of lipid A [Salmonella paratyphi] was investigated in mice in vivo. Two of the 5 synthetic lipid A analogs showed adjuvant activity when they were incorporated into dipalmitoylglycerophosphocholine-cholesterol-dipalmitoylglycerophosphate liposomes sensitized with trinitrophenylaminocaproylglycerophosphoethanolamine. The minimum structure for an adjuvant effect in the liposomal immunogen system was 1-monophosphate of 6-O-(2-deoxy-2-tetradecanoylamino-6-O-tetradecanoyl-D-glucopyranosyl)-2-deoxy-2-tetradecanoyl-amino-3,4-di-O-tetradecanoyl-D-glucopyranose.