Culture and Isolation of Nippostrongylus brasiliensis Infective Larvae for Biochemical Studies

Abstract

Methods for isolating the various stages of parasite life-cycles in large amounts and in a clean condition for biochemical and immunological studies are urgently needed. Among the parasitic nematodes the methods so far developed mainly concern the adult worm, though the eggs of some of the Ascaroidea are notable exceptions. This paper describes adaptations of one of the traditional methods of culturing Nippostrongylus infective larvae which satisfy the needs of biochemical and immunological studies.