CHEMICAL COMPOSITION, ULTRASTRUCTURE AND SOME SEROLOGICAL PROPERTIES OF LIPOPOLYSACCHARIDES FROM LEPTOTRICHIA BUCCALIS

Abstract

Lipopolysaccharide (LPS) was extracted with aqueous phenol from L. buccalis strains L11, ATCC 14201 and ATCC 19616. Virtually all the LPS was found in the H2O phase. LPS could also be extracted with phenol/chloroform/petroleum ether, but not with cold trichloroacetic acid. All LPS preparations contained D-glycero-D-manno-heptose, galactose, glucose, glucosamine n-dodecanoate, 3-hydroxy-n-tetradecanoate and phosphorus. LPS from L11 contained 2-keto-3-deoxyoctonate and an unidentified aldose. LPS from L11 was serologically distinct from LPS of ATCC 14201 and ATCC 19616. LPS from the 2 latter strains crossreacted.