The nirSTBM region coding for cytochrome cd1‐dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono‐, di‐, and tetraheme proteins
- 25 February 1991
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 279 (2), 205-209
- https://doi.org/10.1016/0014-5793(91)80150-2
Abstract
Genes for respiratory nitrite reduction (denitrification) of Pseudomonas stutzeri are clustered within 7 kbp. A 4.6-kbp Hind III-Kpn I fragment carrying nirS, the structural gene for cytochrome cd 1, was sequenced. An open reading frame immediately downstream of nirScodes for a 22.8-kDa protein with four heme c-binding motifs. Mutagenesis of this gene causes an apparent defect in electron donation to cytochrome cd 1. Following this ORF are the structural genes for cytochrome c 552, cytochrome c 551, and ORF5 that codes for a 11.9-kDa monoheme protein. All cytochromes have a signal sequence for protein export.Keywords
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