Chemical Evidence for a Codon‐Induced Allosteric Change in tRNALys Involving the 7‐Methylguanosine Residue 46
Open Access
- 1 July 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 97 (2), 615-621
- https://doi.org/10.1111/j.1432-1033.1979.tb13151.x
Abstract
[32P]tRNALyS, from Escherichia coli, was modified with kethoxal, in the presence and absence of the oligonucleotide codon (A)4. The presence of the codon resulted in a faster modification rate of the tRNA at three guanine sites which were identified by a diagonal fingerprint method. A large increase in the modification rate occurred at the 7-methylguanosine residue 46 (m7G-46) in the presence of the codon; weakly enhanced modification was observed at G-15 and G-57. It is concluded that the formation of a codon-anticodon complex induces, primarily, a confor-mational change involving disruption of the m7G-46 from the m7G-46 · G-22 · C-13 base triple. Subsequently, the guanines of G-15 and G-57, in the D and T loops, respectively, become slightly more reactive, suggesting a weak tendency for these two interacting arms to unfold. The results are interpreted in terms of an equilibrium between two main conformers and a third minor one; the possible significance of these conformers, in protein biosynthesis, is considered.This publication has 32 references indexed in Scilit:
- Effective binding of oligonucleotides to the anticodon of a tRNA without stimulation of tRNA binding to 30 S ribosomesFEBS Letters, 1978
- Codon‐dependent rearrangement of the tertiary structure of tRNAPhe from yeastFEBS Letters, 1977
- Interactions of Yeast tRNAPhe with Ribosomes from Yeast and Escherichia coliEuropean Journal of Biochemistry, 1977
- The kinetics of codon-anticodon interaction in yeast phenylalanine transfer RNAJournal of Molecular Biology, 1975
- Tertiary structure interactions of 7‐methylguanosine in yeast tRNAPhe as studied by borohydride reductionFEBS Letters, 1975
- 3-(3-Amino-3-carboxypropyl)uridine. Structure of the nucleoside in Escherichia coli transfer ribonucleic acid that reacts with phenoxyacetoxysuccinimideBiochemistry, 1974
- On the mRNA induced conformational change of AA-tRNA exposing the T-Ψ-C-G sequence for binding to the 50S ribosomal subunitBiochemical and Biophysical Research Communications, 1974
- The sequence of nucleotides in tRNAIle from E. coli BBiochemical and Biophysical Research Communications, 1971
- Studies on 5 s RNA conformation by partial ribonuclease hydrolysisJournal of Molecular Biology, 1971
- Nucleotide Sequences from Specific Areas of the 16S and 23S Ribosomal RNAs of E. coliEuropean Journal of Biochemistry, 1969