In vitro Assay of Hog Intrinsic Factor with Rat Liver Homogenates

Abstract
A convenient laboratory assay for hog pyloric intrinsic factor is described which involves the uptake of Co60-vitamin B12 upon rat liver homogenates. By means of this in vitro assay the presence of an inhibitory substance was detected in certain crude preparations derived from hog duodenum. These showed a diminished response relative to activity in the Schilling urinary excretion assay.