DNA Synthesis in Subpopulations of Blood Mononuclear Leucocytes in Human Subjects after Vaccination against Yellow Fever
- 1 October 1978
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 8 (4), 339-346
- https://doi.org/10.1111/j.1365-3083.1978.tb00527.x
Abstract
After vaccination of five volunteers with yellow fever live vaccine, blood mononuclear cells were isolated and labelled with 3H-thymidine at intervals. DNA synthesis was measured by scintillation counting and autoradiography of resetted cells. Rosetting with sheep erythrocytes (E-RFC) identified T cells, and such erythrocytes coated with IgM antibodies and complement (EAC-RFC) identified B cells and monocytes. DNA synthesis in the total mononuclear cell fraction, as well as in subfractions enriched in or deprived of E-RFC, displayed a sharp increase on day 10–11 after vaccination, remained high on day 13–14, and then returned to the prevaccination level. There was a corresponding morphological transformation, measured by size distribution and number of nucleoli per cell. The major fraction of DNA-synthesizing cells before, during and after the peak of activity was found among non-rosette-forming cells. However, during the activity peak the numbers and proportion of DNA-synthesizing E-RFC were increased while the response with regard to EAC-RFC was not obvious. Thus within a complex cellular response a transient T-cell response was identified.This publication has 8 references indexed in Scilit:
- Spontaneous 3H·Thymidine Incorporation by Circulating Lymphocytes of Normal SubjectsInternational Archives of Allergy and Immunology, 1977
- Surface Markers on Human B and T LymphocytesScandinavian Journal of Immunology, 1974
- Technical Aspects of the Rosette Tests Used to Detect Human Complement Receptor (B) and Sheep Erythrocyte-Binding (T) LymphocytesThe Journal of Immunology, 1973
- Combined Studies of Complement Receptor and Surface Immunoglobulin-Bearing Cells and Sheep Erythrocyte Rosette-Forming Cells in Normal and Leukemic Human LymphocytesJCI Insight, 1973
- SURFACE MARKERS ON HUMAN T AND B LYMPHOCYTESThe Journal of Experimental Medicine, 1972
- Lymphocyte Mitogenic Factor in ManNature, 1969
- LYMPHOID CELLULAR RESPONSES IN THE BLOOD AFTER IMMUNIZATION IN MANThe Journal of Experimental Medicine, 1969
- Morphological Studies in the Culture of Human Leucocytes with PhytohaemagglutininBritish Journal of Haematology, 1965