Purification of three rat atrial natriuretic factors and their amino acid composition
Open Access
- 12 December 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (2), 286-290
- https://doi.org/10.1016/0014-5793(83)80303-2
Abstract
A natriuretic factor has been described in the specific granules of rat atria. We have purified three factors which seem to be low‐M r peptides. They have been purified by means of acid extraction, octadecyl Sep‐Pak cartridges, and chromatography on Bio‐Gel P‐10, CM Bio‐Gel A, Mono S and reverse‐phase high‐performance liquid chromatography columns. The factors contain 26, 31 and 33 amino acids and may have been partially degraded during isolation. They are all 3 biologically active and the shorter one is the most active with a specific activity of 450000 units/mg.Keywords
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