A MICRO METHOD FOR THE SEPARATION OF 17-KETOSTEROIDS INTO ALPHA AND BETA FRACTIONS
- 1 March 1944
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 34 (3), 175-180
- https://doi.org/10.1210/endo-34-3-175
Abstract
A micro procedure has been described for the separation of urinary 17-ketosteroids into [alpha] and [beta] components, such detns. having been reported to be of clinical significance. The value of the present method lies in its applicability to short-term urine collections (1- to 3-day), and in its direct measurements of both a and j3 fractions with a consequent avoidance of the increased exptl. error inherent in a procedure where the /3 fraction is measured by difference. In expts. on solns. of andro-sterone (an a compound) and of dehydroisoandrosterone (a [beta] compound), 94-102% of the former was recovered when 1.0 to 2.0 mg. were present, and 93-105% of the latter was recovered when 0.12-2.0 mg. were present. A preliminary report is given on the application of the method to urinary extracts from normal and pathological subjects.This publication has 3 references indexed in Scilit:
- ADRENAL CORTICAL HYPERPLASIA WITH VIRILISM: DIAGNOSIS, COURSE AND TREATMENT 12Journal of Clinical Investigation, 1942
- Studies on Prostatic Cancer. V. Excretion of 17-Ketosteroids, Estrogens and Gonadotropins before and after Castration1Journal of Clinical Endocrinology & Metabolism, 1942
- Colorimetric determination of substances containing the grouping —CH2.CO— in urine extracts as an indication of androgen contentBiochemical Journal, 1938