HISTOCHEMICAL DEMONSTRATION OF PROTEIN-BOUND AMINO GROUPS
Open Access
- 1 January 1954
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 2 (1), 29-49
- https://doi.org/10.1177/2.1.29
Abstract
A method for the histochemical demonstration of protein-bound amino groups is described. The method is based upon the reaction of primary amino groups with aldehydes to form Schiff bases. By the use of 3-hydroxy-2-naphthaldehyde, a red to blue azo dye is formed at the sites of reactivity in fixed tissue sections, after coupling with tetrazotized diorthoanisidine. Preparations of the reagent, histochemical procedure, evidence for the specificity of the reaction and the results of its application to normal rat tissues are given.Keywords
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