Solid-Phase Radioimmunoassay of Protein Biosynthesis
- 19 June 1970
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 168 (3938), 1467-1468
- https://doi.org/10.1126/science.168.3938.1467
Abstract
Solid-phase radioimmunoassay was used for measuring the rate of radioisotope incorporation into a single protein species. Radioactive antigen was measured by its binding to specific antiserum covalently linked to insoluble bromoacetylcellulose. The insoluble antigen-antibody complex was collected on filters for counting. The assay is performed in about 4 hours as compared with several days for methods based on the precipitin reaction.Keywords
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