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Effect of Age of Equine Embryos and Method of Transfer on Pregnancy Rate
Home
Publications
Effect of Age of Equine Embryos and Method of Transfer on Pregnancy Rate
Effect of Age of Equine Embryos and Method of Transfer on Pregnancy Rate
MI
M. F. Iuliano
M. F. Iuliano
ES
E. L. Squires
E. L. Squires
VC
V. M. Cook
V. M. Cook
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1 January 1985
journal article
Published by
Oxford University Press (OUP)
in
Journal of Animal Science
Vol. 60
(1)
,
258-263
https://doi.org/10.2527/jas1985.601258x
Abstract
A 2 × 2 cross-classified experiment was conducted to investigate the effect of age of equine embryo (7 vs 8 d postovulation) and method of transfer (surgical vs nonsurgical) on pregnancy rates at 50 d of gestation. Embryos were recovered 7 or 8 d postovulation using a Foley catheter and 3 liters of modified Dulbecco's phosphate-buffered saline (PBS). Upon identification, the embryos were placed in millipore-filtered PBS containing 20% heat- inactivated steer serum and maintained at room temperature until transferred. At the time of recovery, embryos were randomly assigned to be transferred either nonsurgically using a sterile insemination pipette or surgically via a flank incision. For nonsurgical transfer, the embryo was deposited into the uterine body; whereas, in surgical transfer, the embryo was placed in the uterine horn ipsilateral to the corpus luteum. Recovery rates for embryos collected on d 7 (75.5%) or 8 (81.9%) were similar (P>.05). Age of embryo did not affect (P>.05) pregnancy rate. At 50 d, pregnancy rates were 60 and 57% for mares receiving d 7 or 8 embryos. However, more (P<.05) pregnancies were obtained after transfer of embryos surgically (72%) than nonsurgically (45%). More (P<.05) pregnancies were obtained after transfer of d 8 embryos surgically (75%) compared with nonsurgically (40%). Within method of transfer, pregnancy rates were similar (P<.05) for surgical transfer of d 7 and 8 embryos (69 and 75%), but tended (P<.25) to be higher for nonsurgical transfer of d 7 embryos (50%) compared with d 8 embryos (40%). Other factors that affected pregnancy rates after nonsurgical transfer were size of embryo, synchrony of recipients and time of year. Copyright © 1985. American Society of Animal Science. Copyright 1985 by American Society of Animal Science
Keywords
EMBRYOS
SOCIETY
ANIMAL
PBS
PREGNANCY RATES
NONSURGICAL
METHOD OF TRANSFER
SURGICALLY
POSTOVULATION
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Cited by 55 articles