Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid:deoxyribonucleic acid hybridization

Abstract
A method for typing clinical isolates of herpes simplex virus was developed. It utilizes hybridization between unlabeled DNA from infected cultures and tritium-labeled virus DNA, and it can be completed within a day using a single roller-tube culture of the clinical isolate. The data obtained are inherently quantitative, and the method yields unequivocal identification and typing. Thrity-nine coded clinical isolates were all correctly typed by this method.