Alpha-Keto Acids in Vitamin-Free Casein Hydrolyzates (Acid)

Abstract
α-Ketoglutaric and pyruvic acids were isolated as their 2-4-dinitrophenylhydrazones from five different commercial samples of vitamin-free acid hydrolyzates of casein. In addition, one sample yielded traces of α-ketobutyric acid. The hydrazones were converted by hydrogenation to glutamic acid, alanine, and α-aminobutyric acid and identified by paper chromatography.