Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides
- 15 December 1986
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 209 (2), 352-356
- https://doi.org/10.1016/0014-5793(86)81141-3
Abstract
Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-alpha detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.Keywords
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