A RAPID ONE-STEP METHOD FOR THE ISOLATION OF HUMAN-GRANULOCYTES FROM WHOLE-BLOOD
- 1 January 1982
- journal article
- research article
- Vol. 42 (3), 235-238
Abstract
A rapid 1-step method for the separation of granulocytes, mononuclear cells and erythrocytes from peripheral blood using 2 different gradients of Percoll (density solution 1:1.075 kg/l; solution 2; 1.096-1.098 kg/l) is described. The influence of temperature on the density of solution 2 was tested and the variations were of some importance. The average yield of granulocytes from 14 donors was 87.6 .+-. 9.6%, and the purity of the granulocytes was 99.66 .+-. 0.33%.This publication has 3 references indexed in Scilit:
- A rapid method for purification of human granulocytes using percoll®. A comparison with dextran sedimentationJournal of Immunological Methods, 1981
- Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the hypaque-ficoll methodJournal of Immunological Methods, 1980
- Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextranJournal of Immunological Methods, 1979