STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODY II

Abstract

Using fluorescein-labeled anti-Malleomyces pseudomallei globulin, it was possible to identify cells of the homologous species on smears prepared from: (1) Suspensions containing as few as 220 homologous cells per ml. This occurred when suspensions contained only M. pseudomallei, or M. pseudomallei plus other bacterial species in a ratio of as much as 1:10,000,000. (2) Experimentally seeded soil. (3) Material collected from surfaces previously sprayed with suspensions of the organism. (4) Tissues of experimentally infected animals.