Simultaneous Determination of Allantoin and Creatinine in Urine by a Rapid Reversed-Phase Liquid-Chromatographic Method
- 1 May 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 15 (8), 1337-1350
- https://doi.org/10.1080/10826079208018291
Abstract
A simple, rapid, reproducible RP-HFLC method for a simultaneous analysis of allantoin and creatinine in sheep urine, is described. Separation was achieved on a Novapak-C18 (3.9×300 mm) column by isocratic elution. Quantitation and detection limits were determined. Detection was effected at 218 nm. Retention times of allantoin and creatinine were 4.4 and 5. 8 min, respectively. The proposed RP-HPLC method was verified for linearity, accuracy, precision and applicability.Keywords
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