DETERMINATION OF PROTEIN AND LIPID LOST DURING OSMIC ACID FIXATION OF TISSUES AND CELLULAR PARTICULATES
- 1 March 1957
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 5 (2), 178-181
- https://doi.org/10.1177/5.2.178
Abstract
Heart and kidney fragments and liver mitochondria and microsomes were prepared for electron microscopy by standard methods. The fixative, dehydration and embedding mediums each were analyzed separately for protein and lipid removed. It was demonstrated that protein and lipid were lost from mitochondria and microsomes and that protein was lost from tissue fragments during their preparation for electron microscopic examination. The significance of these results with respect to investigations concerned with the correlation of function to morphology is discussed.Keywords
This publication has 4 references indexed in Scilit:
- LIVER MICROSOMESThe Journal of cell biology, 1956
- MORPHOLOGY AND ATP-ASE OF ISOLATED MITOCHONDRIAThe Journal of cell biology, 1955
- Studies on the chemical composition of nuclei and cytoplasmic granulesArchives of Biochemistry and Biophysics, 1955
- A STUDY OF FIXATION FOR ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1952