The biosynthesis of dentine γ-carboxyglutamic acid-containing proteins by rat incisor odontoblasts in organ culture
- 14 November 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 216 (2), 249-257
- https://doi.org/10.1042/bj2160249
Abstract
Rat odontoblasts synthesize the secrete .gamma.-carboxyglutamic acid(Gla)-containing proteins into dentine after organ culture in the presence of radiolabeled amino acid precursors. Purified dentine Gla-containing protein from rat incisors was used as antigen to prepare rabbit antisera as a probe of dentine Gla-containing-protein biosynthesis in organ cultures of dentine (rat incisor) and bone (rat calvaria). Use of the antiserum also pointed out the cross-reactivity of a high-MW glycoprotein present within the dentine matrix. The present results are significant in identifying dentine Gla-containing protein as endogenous to mineralizing dentine and may relate to the commonality between calcifying connective tissues in general.This publication has 3 references indexed in Scilit:
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- Membrane biogenesis: cotranslational integration of the bacteriophage f1 coat protein into an Escherichia coli membrane fraction.Proceedings of the National Academy of Sciences, 1979
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