THE SURFACE CHARGE OF RAT LIVER MITOCHONDRIA AND THEIR MEMBRANES
Open Access
- 1 July 1970
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 46 (1), 137-150
- https://doi.org/10.1083/jcb.46.1.137
Abstract
The surface charge of intact mitochondria and submitochondrial particles was examined by the technique of preparative free flow electrophoresis. When submitochondrial preparations obtained by a swelling-contraction procedure were examined with this technique, two fractions were observed. One of these fractions exhibited the same electrophoretic properties as intact mitochondria, which indicated that it was derived from the outer limiting membrane of the mitochondrion. This fraction was found to contain the enzymes monoamine oxidase and rotenone-insensitive NADH-cytochrome c reductase which have been reported to be localized in the outer mitochondrial membrane. The other fraction exhibited an electrophoretic mobility which was different from that of intact mitochondria, and this fraction contained enzymes characteristic of the inner membrane-matrix fraction such as soluble and particulate enzymes of the Krebs cycle. Microsomes exhibited an electrophoretic mobility which was almost identical with that of the outer mitochondrial membrane. In addition to resolving the localization of enzymes in mitochondrial membranes, these data indicate that the outer limiting membrane of the mitochondrion is the sole determinant of the surface charge of mitochondria.Keywords
This publication has 22 references indexed in Scilit:
- Enzyme localization in the inner and outer mitochondrial membranesBiochemical and Biophysical Research Communications, 1968
- The sub-mitochondrial localization of monoamine oxidase in rat liver and brainBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- Some biochemical and X-ray diffraction studies of mitochondrial outer membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- Séparation des membranes mitochondriales. Purification et caractérisation enzymatique de la membrane externeBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- Incorporation of amino acids into the outer and inner membrane of isolated rat liver mitochondriaBiochemical and Biophysical Research Communications, 1967
- THE SUBMITOCHONDRIAL LOCALIZATION OF MONOAMINE OXIDASEThe Journal of cell biology, 1967
- AN ELECTRON-TRANSPORT SYSTEM ASSOCIATED WITH THE OUTER MEMBRANE OF LIVER MITOCHONDRIAThe Journal of cell biology, 1967
- BIOCHEMICAL AND ULTRASTRUCTURAL PROPERTIES OF OSMOTICALLY LYSED RAT-LIVER MITOCHONDRIAThe Journal of cell biology, 1966
- The membrane systems of the mitochondrionArchives of Biochemistry and Biophysics, 1966
- CHARACTERISTICS OF ISOLATED AND PURIFIED PREPARATIONS OF THE OUTER AND INNER MEMBRANES OF MITOCHONDRIA*Annals of the New York Academy of Sciences, 1966