Photocrosslinkers illuminate interactions in living cells
- 23 April 2008
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Molecular BioSystems
- Vol. 4 (6), 473-480
- https://doi.org/10.1039/b803218a
Abstract
Transient and low-affinity interactions among macromolecules underlie many physiological events. Often, these interactions are difficult to study because they are not maintained when the participating molecules are removed from their cellular context. To circumvent this challenge, crosslinking reagents can be used to introduce covalent bonds between interacting macromolecules. Photoactivatable crosslinkers are particularly attractive because they allow crosslinking to proceed in time- and location-specific ways. Once the interacting partners have been crosslinked, they can be isolated and then analyzed by mass spectrometry or other analytical techniques to determine the identity of the interacting molecules and to pinpoint the interacting regions. This review highlights recent methodological developments that make it possible to introduce photocrosslinking groups into polypeptides or glycans as they are synthesized in cells. We also describe how these methods offer a non-invasive way to study macromolecular interactions in a native context.Keywords
This publication has 64 references indexed in Scilit:
- An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approachNature Protocols, 2006
- Methionine acts as a “magnet” in photoaffinity crosslinking experimentsFEBS Letters, 2006
- Homomultimeric complexes of CD22 in B cells revealed by protein-glycan cross-linkingNature Chemical Biology, 2005
- Expanding the Diversity of Unnatural Cell‐Surface Sialic AcidsChemBioChem, 2004
- Functional organization of the yeast proteome by systematic analysis of protein complexesNature, 2002
- Survey of four different photoreactive moieties for DNA photoaffinity labeling of yeast RNA polymerase III transcription complexesNucleic Acids Research, 1998
- Benzophenone Photophores in BiochemistryBiochemistry, 1994
- NEW PHOTOLABELING AND CROSSLINKING METHODSAnnual Review of Biochemistry, 1993
- ARYL AZIDES AS PROTEIN PHOTOLABELS: ABSORPTION SPECTRAL PROPERTIES AND QUANTUM YIELDS OF PHOTODISSOCIATIONPhotochemistry and Photobiology, 1984
- Photogenerated reagents for biological receptor-site labelingAccounts of Chemical Research, 1972