Recombinant aequorin as a probe for cytosolic free Ca2+ in Escherichia coli
Open Access
- 6 May 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 282 (2), 405-408
- https://doi.org/10.1016/0014-5793(91)80524-7
Abstract
We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant acquorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement.Keywords
This publication has 8 references indexed in Scilit:
- Adenylate cyclase activity in Cyanobacteria: activation by Ca2+-calmodulin and a calmodulin-like activityBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- THE ENZYMOLOGY AND MOLECULAR BIOLOGY OF THE Ca2+‐ACTIVATED PHOTOPROTEIN, AEQUORINPhotochemistry and Photobiology, 1989
- Maintenance of intracellular calcium in Escherichia coli.Journal of Biological Chemistry, 1987
- A bacterial calcium-binding protein homologous to calmodulinNature, 1987
- Chemiluminescence as an Analytical Tool in Cell Biology and MedicineMethods of Biochemical Analysis, 1985
- Immunoradiometric assay for human complement component C9 utilising monoclonal antibodiesClinica Chimica Acta; International Journal of Clinical Chemistry, 1983
- Measurement of changes in cytoplasmic free Ca2+ in fused cell hybridsNature, 1982
- [31] Practical aspects of the use of aequorin as a calcium indicator: Assay, preparation, microinjection, and interpretation of signalsPublished by Elsevier ,1978