Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (12), 3773-3784
- https://doi.org/10.1093/nar/6.12.3773
Abstract
Lecithin and lecithin/cholesterol liposomes formed in aqueous solutions of DNA entrap covalently closed circular, open circular and linear DNA molecules of size up to at least 13 kilobases. The sequestered DNA molecules are efficiently protected against exogenous deoxyribonuclease action although nicking and linearization of circular DNA can be observed. The size of these liposomes ranges from approximately 0.5 to 7.5 mu with an average of 2.5--4 mu. DNA filled liposomes strongly interact with plant protoplasts under conditions inducing protoplast fusion. Results suggest that sequestered plasmid DNA can be transferred to protoplast nuclei.Keywords
This publication has 18 references indexed in Scilit:
- Binding and entrapment of high molecular weight DNA by lecithin liposomesFEBS Letters, 1978
- Translation of rabbit globin mRNA introduced by liposomes into mouse lymphocytesNature, 1978
- Inhibition of Deoxyribonuclease Activity in the Medium Surrounding Plant ProtoplastsPlant Physiology, 1978
- Incorporation and maintenance of recombinant‐DNA plasmid vehicles pBR313 and pCR1 in plant protoplastsFEBS Letters, 1978
- PROSPECTS FOR PLANT GENOME MODIFICATION BY NONCONVENTIONAL METHODSAnnual Review of Genetics, 1977
- Fate of bacterial plasmid DNA during uptake by barley protoplastsFEBS Letters, 1977
- Quantitative Analysis of the Fate of Exogenous DNA in Nicotiana ProtoplastsPlant Physiology, 1977
- Uptake of bacterial DNA by Chlamydomonas reinhardiMutation Research, 1975
- DNA-mediated genetic correction of thiamineless Arabidopsis thalianaNature, 1974
- A fluorescent complex between ethidium bromide and nucleic acidsJournal of Molecular Biology, 1967